GadE (YhiE) activates glutamate decarboxylase-dependent acid resistance in Escherichia coli K-12

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GadE (YhiE) activates glutamate decarboxylase-dependent acid resistance in Escherichia coli K-12.

Commensal and pathogenic strains of Escherichia coli possess three inducible acid resistance systems that collaboratively protect cells against acid stress to pH 2 or below. The most effective system requires glutamate in the acid challenge media and relies on two glutamate decarboxylases (GadA and B) combined with a putative glutamate:gamma-aminobutyric acid antiporter (GadC). A complex networ...

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GadE (YhiE): a novel activator involved in the response to acid environment in Escherichia coli.

In several Gram-positive and Gram-negative bacteria glutamate decarboxylases play an important role in the maintenance of cellular homeostasis in acid environments. Here, new insight is brought to the regulation of the acid response in Escherichia coli. Overexpression of yhiE, similarly to overexpression of gadX, a known regulator of glutamate decarboxylase expression, leads to increased resist...

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Genetic and physiological analysis of glutamate decarboxylase in Escherichia coli K-12.

No correlation was found between glutamate decarboxylase (GAD) activity and the ability of Escherichia coli K-12 strains to grow on glutamate. A gene, gad, determining GAD activity maps near gltC, which controls glutamate permease.

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actIvators of Glutamate-dependent acId resIstance sYstem allevIate deleterIous effects of YIdc depletIon In EschErichia coli

1department of molecular microbiology, Institute of molecular cell biology, vu university amsterdam, the netherlands, 2swammerdam Institute for life sciences, molecular microbial physiology, university of amsterdam, amsterdam, the netherlands, 3Istituto di biologia e patologia molecolari, cnr, dipartimento di scienze biochimiche, università di roma sapienza, roma, Italy, 4 department of microbi...

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Thermoregulation-dependent expression of Yersinia enterocolitica protein 1 imparts serum resistance to Escherichia coli K-12.

Resistance to the bactericidal action of normal human serum is one of the characteristics of virulent Yersinia enterocolitica. This property is attributable to the virulence plasmid harbored by pathogenic strains of the species. Serum resistance in Y. enterocolitica is thermoregulated, and its expression correlates well with the presence of virulence plasmid-encoded outer membrane proteins. To ...

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ژورنال

عنوان ژورنال: Molecular Microbiology

سال: 2003

ISSN: 0950-382X,1365-2958

DOI: 10.1046/j.1365-2958.2003.03633.x